Aim: To evaluate and compare the microhardness of artificially demineralized human enamel treated with three different remineralizing agents. Materials and methods: Twenty-five intact caries free maxillary premolar were taken for the study. The tooth was decoronated 2 mm below cementoenamel junction (CEJ). Enamel samples of 2 mm width were prepared, the enamel slices were taken from the buccal and lingual surfaces of the teeth using a diamond disc. Following sample preparation, windows were created with a dimension of 5 mm × 5 mm. Adhesive tape was placed over it to resist acid attack. The specimens were demineralized using a demineralizing solution for ten days and then subjected to microhardness testing. Teeth were divided into five groups. • Group 1: Negative control in which no therapy was done • Group 2: Demineralized enamel specimen • Group 3: Remineralization with potassium nitrate containing toothpaste • Group 4: Remineralization with casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) containing toothpaste, • Group 5: Remineralization with hydroxyapatite containing toothpaste. The remineralizing agents were applied using cotton applicator tips for 3 minutes twice daily and they were washed under distilled water. The samples were then stored in artificial saliva for 10 days and subjected to microhardness testing. Statistical analysis: One-way analysis of variance (ANOVA) test. A post hoc comparison showed that done using a paired t-test. Results: The result showed that there has been statistically significant difference in the microhardness for control samples and experimental samples. Within the experimental samples, group which used hydroxyapatite paste as remineralizing paste showed better results when compared to paste which contain calcium and potassium ions and sodium nitrate.