Citation Information :
Ummer S, Dhanapal P, Charlie K, Joseph A. Evaluation and Comparison of Three Chemical Agents with a New Herbal Agent for Disinfection of Gutta-percha Cones: An in vitro Study. Cons Dent Endod J 2016; 1 (2):33-37.
Aims: To evaluate the effectiveness of grape seed extract (GSE) as a gutta-percha disinfectant and to compare the efficacy with 5% sodium hypochlorite, 2.5% sodium hypochlorite, and 2% chlorhexidine against Bacillus subtilis.
Materials and methods: Minimum inhibitory concentration and minimum bactericidal concentration of GSE were calculated by serial dilution and well-diffusion method. Five hundred gutta-percha cones of ISO size 25 were divided into 10 equal groups (n = 50). The 10 groups of gutta-percha cones were inoculated with cultured Bacillus species and incubated for 72 hours for allowing bacterial growth. Each group was then treated with the test solutions for 1 and 5 minutes. The treated groups of gutta-percha cones were then incubated in brain heart infusion agar allowing for bacterial growth, which were later analyzed by the turbidity of the medium. The results were statistically analyzed.
Results: Gutta-percha cones when treated with 2% chlorhexidine gluconate for 1 and 5 minutes showed the most inhibiting activity against B. subtilis. Grape seed extract was found to have limited activity against B. subtilis in both 1- and 5-minute interval. Both concentrations of NaOCl, 2.5 and 5%, showed reduced activity against B. subtilis.
Conclusion: Grape seed extract though has antibacterial activity, when used as gutta-percha disinfectant, was found to be less effective than chlorhexidine gluconate against B. subtilis.
Clinical significance: Different methods of gutta-percha cone disinfection have been advocated and GSE for gutta-percha cone disinfection was attempted owing to its herbal antibacterial nature.
Hurtt CA, Rossman LE. The sterilization of endodontic hand files. J Endod 1996 Jun;22(6):321-322.
Namazikhah MS, Sullivan DM, Trnavsky GL. Gutta-percha: a look at the need for sterilization. J Calif Dent Assoc 2000 Jun;28(6):427-432.
Gomes BP, Vianna ME, Matsumoto CU, Rossi Vde P, Zaia AA, Ferraz CC. Disinfection of gutta-percha cones with chlorhexidine and sodium hypochlorite. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2005 Oct;100(4):512-517.
Zand V, Salem-Milani A, Shahi S, Akhi MT, Vazifekhah S. Efficacy of different concentrations of sodium hypochlorite and chlorhexidine in disinfection of contaminated Resilon cones. Med Oral Patol Oral Cir Buccal 2012 Mar 1;17(2):e352-e355.
Pradeep K, Kidiyoor KH, Jain P, Rao N. Chair side disinfection of gutta-percha points – An in vitro comparative study between 5 different agents at different concentrations. Endodontology 2013 Jun;25:73.
Pang NS, Jung IY, Bae KS, Baek SH, Lee WC, Kum KY. Effects of short-term chemical disinfection of gutta-percha cones: identification of affected microbes and alterations in surface texture and physical properties. J Endod 2007 May;33(5):594-598.
Seabra Pereira OL, Siqueira JF Jr. Contamination of gutta-percha and Resilon cones taken directly from the manufacturer. Clin Oral Investig 2010 Jun;14(3):327-330.
Siqueira JF Jr, da Silva CH, Cerqueira M das D, Lopes HP, de Uzeda M. Effectiveness of four chemical solutions in eliminating Bacillus subtilis spores on gutta-percha cones. Endod Dent Traumatol 1998 Jun;14(3):124-126.
Linke HAB, Chohayeb AA. Effective surface sterilization of gutta-percha points. Oral Surg Oral Med Oral Pathol 1983 Jan;55(1):73-77.
Cardoso C, Kotaka C, Redmerski R, Guilhermetti M, Queiroz AF. Rapid decontamination of gutta-percha cones with sodium hypochlorite. J Endod 1999 Jul;25(7):498-501.
Ozalp N, Okte Z, Ozcelik B. The rapid sterilization of guttapercha cones with sodium hypochlorite and glutaraldehyde. J Endod 2006 Dec;32(12):1202-1204.
de Souza RE, de Souza EA, Sousa-Neto MD, Pietro RC. In vitro evaluation of different chemical agents for the decontamination of gutta-percha cones. Pesqui Odontol Bras 2003 Jan-Mar;17(1):75-77.
Redmerski R, Bulla JR, Moreno T, Garcial LB, Cardoso C. Disinfection of gutta-percha cones with chlorhexidine. Braz J Microbiol 2007 Oct-Dec;38(4):649-655.
Monagas M, Gomez-Cordoves C, Bartolome B, Laureano O, Ricardo da Silva JM. Monomeric, oligomeric, and polymeric flavan-3-ol composition of wines and grapes from Vitis vinifera L. Cv. Graciano, Tempranillo, and Cabernet Sauvignon. J Agric Food Chem 2003 Oct;51(22):6475-6481.
Jayaprakasha GK, Selvi T, Sakariah KK. Antibacterial and antioxidant activities of grape (Vitis vinifera) seed extracts. Food Res Int 2003;36:117-122.
Nakamura Y, Tsuji S, Tonogai Y. Analysis of proanthocyanidins in grape seed extracts, health foods and grape seed oils. J Health Sci 2003;49(1):45-54.
Karou D, Dicko MH, Simpore J, Traore AS. Antioxidant and antibacterial activities of polyphenols from ethnomedicinal plants of Burkina Faso. Afr J Biotechnol 2005;4(8):823-828.
Cardoso CL, Redmerski R, Bittencourt NLR, Kotaka CR. Effectiveness of different chemical agents in rapid decontamination of guttapercha cones. Braz J Microbiol 2000 Jan-Mar;31(1):67-71.